Rabbit anti‑Salmon Collagen I Polyclonal RW-C744038-0.1

Antibody: Collagen I Rabbit anti-Salmon Polyclonal Antibody, Application: IHC-P, IF, ELISA, RIA, Reactivity: Salmon, Goldfish, Sole, Tuna, Format: Unconjugated, Unmodified, Descriptio: Collagen I antibody RW-C744038 is an unconjugated rabbit polyclonal antibody to Collagen I from salmon. It is reactive with goldfish, salmon, sole and other species. Validated for ELISA, IF, IHC and RIA., Targe: Salmon Collagen I, Hos: Rabbit, Reactivit: Salmon, Goldfish, Sole, Tuna (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Modification: Unmodified, Immunoge: Purified collagen type I from fish skin, Application: IHC - Paraffin (1:500)
Immunofluorescence (1:80)
ELISA (1:100 - 1:200)
Radioimmunoassay, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Salmon (Salmon salar) Collagen type I., Presentatio: PBS, Reconstitutio: 0.5 ml Reconstitute in sterile distilled water., Storag: Store at -20°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.