Rabbit anti‑Cucurbita AAO / L-Ascorbate Oxidase IgG Polyclonal RW-C209666-100
Antibody: AAO / L-Ascorbate Oxidase Rabbit anti-Cucurbita Polyclonal (Lyophilized) (Biotin) Antibody, Application: WB, IP, ELISA, Reactivity: Cucurbita, Format: Biotin, Lyophilized, Descriptio: L-Ascorbate Oxidase antibody RW-C209666 is a biotin-conjugated rabbit polyclonal antibody to cucurbita L-Ascorbate Oxidase (AAO). Validated for ELISA, IP and WB., Targe: Cucurbita AAO / L-Ascorbate Oxidase, Synonym: AAO, Hos: Rabbit, Reactivit: Cucurbita (tested or 100% immunogen sequence identity), Clonalit: IgG Polyclonal, Conjugation: Biotin, Purificatio: Delipidation, salt fractionation and ion exchange chromatography followed by dialysis., Modification: Lyophilized, Immunoge: Ascorbate Oxidase [cucurbita]., Specificit: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidase from other sources may occur but have not been specifically determined., Application: Western blot (1:500 - 1:5000)
Immunoprecipitation (1:100)
ELISA (1:2500 - 1:15000), Usag: Anti-Ascorbate Oxidase has been assayed against 1.0 ug of Ascorbate Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2000 to 1:10000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested., Presentatio: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, Reconstitutio: Reconstitute in 100 µL of deionized water., Storag: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About AAO / L-Ascorbate Oxidas: NCBI: P24792