Rabbit anti‑Human ABCA1 Polyclonal RW-B3038-0.1

Antibody: ABCA1 Rabbit anti-Human Polyclonal (aa1100-1300) Antibody, Application: IHC, IHC-P, WB, Reactivity: Human, Mouse, Rat, Dog, Hamster, Pig, Chicken, Mustelid, Format: Unconjugated, Unmodified, Other formats: DY650 DY488 DY550 Biotin, Descriptio: ABCA1 (ATP-binding cassette subfamily A member 1, CERP) is a transporter protein that modulates phospholipid homeostasis, cellular cholesterol. ABCA1 is necessary for proper cholesterol metabolism and transport in the central nervous system. It has highest expression in the liver, adipose tissue and the small intestine, and is otherwise found throughout the body. Mutations in ABCA1 lead to the rare Tangier’s disease, described by a massive reduction in high density lipoprotein in the body and which can lead to neuropathy and cardiovascular disease. Downregulation of ABCA1 leads to atherogenesis through a disruption of cholesterol transport within cells and is a factor in the pathogenesis of atherosclerosis and macular degeneration. Upregulation of ABCA1 has been found to reduce amyloid deposition in mouse models of Alzheimer’s disease. In immunohistochemistry of normal tissue, ABCA1 has membranous and cytoplasmic positivity in tissues throughout the body.

References: Cell Metabolism. 17 (4): 549–61, PMID: 23562078; J Clin Invest. 2008;118(2):671–682, DOI: doi.org/10.1172/JCI33622; J Neurosci. 2009 Mar 18; 29(11): 3579–3589, PMID: 19295162, Targe: Human ABCA1, Synonym: ABCA1 | ABC-1 | ABC1 | CERP | HDLDT1 | Tangier disease | ATP-binding cassette 1 | Td | Membrane-bound | TGD, Hos: Rabbit, Reactivit: Human, Mouse, Rat, Dog, Hamster, Pig, Chicken, Mustelid (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated. Also available conjugated with DY650, DY488, DY550, Biotin., Purificatio: Immunogen affinity purified, Modification: Unmodified, Immunoge: Partial peptide sequence (peptide used resides somewhere between a. a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information, Epitop: aa1100-1300, Specificit: This antibody is specific for ABCA1., Application: IHC
IHC - Paraffin (5 µg/ml)
Western blot, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Immunohistochemistry: RW-B3038 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for RW-B3038 was determined to be 5 ug/ml., Presentatio: PBS, 0.05% Sodium Azide, Storag: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ABCA: Uniprot: O95477 NCBI: NM_005502 NP_005493.2