Mouse anti‑Arabidopsis Actin IgM,k Monoclonal RW-B14148-0.1

Antibody: Actin Mouse anti-Arabidopsis Monoclonal (mAbGEa) Antibody, Application: IHC-P, ICC, IF, WB, ELISA, Reactivity: Arabidopsis, Human, Mouse, Rat, Bovine, Rabbit, Sheep, Xenopus, Zebrafish, Drosophila, Yeast, Fungus, Hamster-Chinese, Plant, Protozoa, Format: Unconjugated, Unmodified, Descriptio: Actins are ubiquitously expressed proteins that are a major component of the cytoskeleton. Actin is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues. It regulates motility and controls cells structure in both muscle and non-muscle cells (where it is present in the cytoplasm as well). Pan-actin antibodies can be used to identify actin expression and localization in normal and disease states. Pan-actin antibodies can be used to label myotubes, myoblasts and fibroblasts. They are useful for determining total actin levels to compare against the differential expression of specific actins in normal versus diseased tissue, such as kidney tissue in diabetes.

References: Cytoskeleton (Hoboken). 2010 Nov;67(11):729-43, PMID: 20862689; Clin Proteom 8, 15 (2011) doi:10.1186/1559-0275-8-15; Molecular & Cellular Proteomics. 2012. 11 (8): 255-271, DOI: 10.1074/mcp.M111.015099, Targe: Arabidopsis Actin, Hos: Mouse, Reactivit: Arabidopsis, Human, Mouse, Rat, Bovine, Rabbit, Sheep, Xenopus, Zebrafish, Drosophila, Yeast, Fungus, Hamster-Chinese, Plant, Protozoa (tested or 100% immunogen sequence identity), Clonalit: IgM,k Monoclonal, Clon: mAbGEa, Conjugation: Unconjugated, Purificatio: Purified IgM, Modification: Unmodified, Immunoge: Purified recombinant Arabidopsis Actin protein [UniProt# P0CJ46], Specificit: Arabidopsis Actin. Arabidopsis Actin. Reacts with Actin 1, 2, 3, 4, 7, 8, 11 and 12., Application: IHC - Paraffin (5 µg/ml)
ICC (1:20 - 1:100)
Immunofluorescence (1:20 - 1:100)
Western blot (1:25)
ELISA (1:100 - 1:2000), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: This Actin Antibody (mAbGEa) is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band is seen at ~45 kDa representing Actin. In IHC-P and ICC/IF, cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point., Presentatio: PBS, 0.05% Sodium Azide, Storag: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.