Mouse anti‑Human ACE / CD143 IgG1 Monoclonal RW-B2558-50
Antibody: ACE / CD143 Mouse anti-Human Monoclonal Antibody, Application: IHC, IHC-P, WB, Reactivity: Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit, Format: Unconjugated, Unmodified, Descriptio: ACE (Angiotensin-converting enzyme, CD143) is an enzyme involved in catalyzing the conversion of angiotensin I into the physiologically active angiotensin II. It has a central role in the renin-angiotensin system, which is involved in regulating blood pressure and fluid volume in the body. The 287 bp Alu repeat element in this gene is correlated with levels of circulating ACE enzyme and with cardiovascular patho-physiologies. Variants in ACE are also associated with Alzheimer’s disease, where deregulated levels of the protein have been found. In immunohistochemistry, ACE has positive staining in the lung capillary endothelium, basal ganglia neuropil, granulosa cells, Leydig cells, proximal renal tubule brush borders, the uterus, male genitalia, and endothelial cells of small or medium arteries.
References: Am J Transl Res. 2011 Jan 1; 3(1): 73–80, PMID: 21139807; Alzheimers Res Ther. 2014; 6(3): 27, PMID: 24987467, Targe: Human ACE / CD143, Synonym: ACE | ACE1 | Angiotensin-converting enzyme | CD143 | CD143 antigen | Carboxycathepsin | DIPEPTIDYL CARBOXYPEPTIDASE | DCP | Dipeptidyl carboxypeptidase I | ICH | Kininase II | MVCD3 | Peptidyl-dipeptidase A | Peptidase P | DCP1 | Dipeptidyl carboxypeptidase 1 | Testicular ECA, Hos: Mouse, Reactivit: Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity), Clonalit: IgG1 Monoclonal, Conjugation: Unconjugated, Purificatio: Purified, Modification: Unmodified, Immunoge: Angiotensin-converting enzyme (denatured) from human kidney, Specificit: Denatured Angiotensin-converting enzyme (ACE). Species cross-reactivity: Human, monkey, rabbit, bovine, canine, feline, guinea pig, rat and mouse., Application: IHC
IHC - Paraffin (10 µg/ml)
Western blot, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Immunohistochemistry: RW-B2558 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for RW-B2558 was determined to be 10 ug/ml., Presentatio: No preservatives added, Storag: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ACE / CD14: Uniprot: P12821 NCBI: NM_000789 NP_000780.1